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Biologic characteristics of platelet rich plasma and platelet rich fibrin : A review

Farzaneh Lal Alizade, Mustafa Kazemi, Sahar Irani, Mehdi Sohrabi


The recent development of platelet concentrate for surgical use is an evolution of the fibrin glue technologies used since many years. Fibrin is a biologic glue that compact platelet clusters during coagulation process fibrin glues are biologic products that their local application has been used to decrease bleeding and accelerate tissue healing in the past three decades. Fibrin glue prepared based on a natural biologic mechanism (polymerization of fibrin during homeostasis) that reinforced by an artificial way. However, they were ever criticized because were blood derived products and had a high risk of viral infection the production of these products is based on the mixture of two plasma components including fibrinogen and thrombin. Platelet-rich plasma (PRP) is a fibrin matrix in which platelet cytokines, growth factors, and cells are trapped and may be released after a certain time. Choukroun et al. used platelet rich fibrin (PRF) protocol in oral and maxillofacial surgery to improve bone healing. Autologous PRF is considered to be a healing biomaterial, and presently, studies have shown its application in various disciplines of dentistry. PRF is the second generation of platelet concentrates which allows fibrin membranes to get enriched with platelets and growth factors, starting from an anticoagulant free blood harvest. PRF is similar to a fibrin network that allows cell migration and proliferation, and consequently, a more efficient cicatrization. Many growth factors, such as platelet-derived growth factor and transforming growth factor, are released from PRF. One of the major differences between PRF and PRP is the different polymerization that is responsible for the different biologic properties. PRF released autologous growth factors gradually and expressed stronger and more durable effect on proliferation and differentiation, which means that PRF could stimulate the surrounding environment to a more rapid wound healing. This review aims to evaluate the effect of biologic characteristics of fibrin glues, PRP, and PRF.


Fibrin; platelet rich fibrin; platelet rich plasma

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Matras H. Effect of various fibrin preparations on reimplantations in the rat skin. Osterr Z Stomatol 1970;67:338-59.

Gibble JW, Ness PM. Fibrin glue: The perfect operative sealant? Transfusion 1990;30(8):741-7.

Clark RA. Fibrin and wound healing. Ann N Y Acad Sci 2001;936:355-67.

van Hinsbergh VW, Collen A, Koolwijk P. Role of fibrin matrix in angiogenesis. Ann N Y Acad Sci 2001;936:426-37.

Vinazzer H. Fibrin sealing: Physiologic and biochemical background. Facial Plast Surg 1985;2:291-5.

Whitman DH, Berry RL, Green DM. Platelet gel: An autologous alternative to fibrin glue with applications in oral and maxillofacial surgery. J Oral Maxillofac Surg 1997;55:1294-9.

Saltz R, Sierra D, Feldman D, Saltz MB, Dimick A, Vasconez LO. Experimental and clinical applications of fibrin glue. Plast Reconstr Surg 1991;88:1005-15.

Hotz G. Alveolar ridge augmentation with hydroxylapatite using fibrin sealant for fixation. Part I: An experimental study. Int J Oral Maxillofac Surg 1991;20:204-7.

Hotz G. Alveolar ridge augmentation with hydroxylapatite using fibrin sealant for fixation. Part II: Clinical application. Int J Oral Maxillofac Surg 1991;20:208-13.

Bonucci E, Marini E, Valdinucci F, Fortunato G. Osteogenic response to hydroxyapatite-fibrin implants in maxillofacial bone defects. Eur J Oral Sci 1997;105:557-61.

Matras H. Fibrin sealant in maxillofacial surgery. Development and indications. A review of the past 12 years. Facial Plast Surg 1985;2:297-313.

Matras H. Fibrin seal: The state of the art. J Oral Maxillofac Surg 1985;43:605-11.

Soffer E, Ouhayoun JP, Anagnostou F. Fibrin sealants and platelet preparations in bone and periodontal healing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003;95:521-8.

Giannobile WV. Periodontal tissue engineering by growth factors. Bone 1996;19 1 Suppl:23S-37.

Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998;85:638-46.

Weibrich G, Kleis WK, Hafner G, Hitzler WE, Wagner W. Comparison of platelet, leukocyte, and growth factor levels in point-of-care platelet-enriched plasma, prepared using a modified Curasan kit, with preparations received from a local blood bank. Clin Oral Implants Res 2003;14:357-62.

Arpornmaeklong P, Kochel M, Depprich R, Kübler NR, Würzler KK. Influence of platelet-rich plasma (PRP) on osteogenic differentiation of rat bone marrow stromal cells. An in vitro study. Int J Oral Maxillofac Surg 2004;33:60-70.

Carter CA, Jolly DG, Worden CE Sr, Hendren DG, Kane CJ. Platelet-rich plasma gel promotes differentiation and regeneration during equine wound healing. Exp Mol Pathol 2003;74:244-55.

Yamada Y, Ueda M, Hibi H, Nagasaka T. Translational research for injectable tissue-engineered bone regeneration using mesenchymal stem cells and platelet-rich plasma: From basic research to clinical case study. Cell Transplant 2004;13:343-55.

Yamada Y, Ueda M, Naiki T, Takahashi M, Hata K, Nagasaka T. Autogenous injectable bone for regeneration with mesenchymal stem cells and platelet-rich plasma: Tissue-engineered bone regeneration. Tissue Eng 2004;10:955-64.

Wang HL, Avila G. Platelet rich plasma: Myth or reality? Eur J Dent 2007;1:192-4.

Bennett NT, Schultz GS. Growth factors and wound healing: Biochemical properties of growth factors and their receptors. Am J Surg 1993;165:728-37.

Sánchez AR, Sheridan PJ, Kupp LI. Is platelet-rich plasma the perfect enhancement factor? A current review. Int J Oral Maxillofac Implants 2003;18:93-103.

El-Sharkawy H, Kantarci A, Deady J, Hasturk H, Liu H, Alshahat M, et al. Platelet-rich plasma: Growth factors and pro- and anti-inflammatory properties. J Periodontol 2007;78:661-9.

Fernández-Barbero JE, Galindo-Moreno P, Avila-Ortiz G, Caba O, Sánchez-Fernández E, Wang HL. Flow cytometric and morphological characterization of platelet-rich plasma gel. Clin Oral Implants Res 2006;17:687-93.

Anitua E. Plasma rich in growth factors: Preliminary results of use in the preparation of future sites for implants. Int J Oral Maxillofac Implants 1999;14:529-35.

Weibrich G, Kleis WK, Kunz-Kostomanolakis M, Loos AH, Wagner W. Correlation of platelet concentration in platelet-rich plasma to the extraction method, age, sex, and platelet count of the donor. Int J Oral Maxillofac Implants 2001;16:693-9.

Jakse N, Tangl S, Gilli R, Berghold A, Lorenzoni M, Eskici A, et al. Influence of PRP on autogenous sinus grafts. An experimental study on sheep. Clin Oral Implants Res 2003;14:578-83.

Freymiller EG, Aghaloo TL. Platelet-rich plasma: Ready or not? J Oral Maxillofac Surg 2004;62:484-8.

Petrungaro PS. Using platelet-rich plasma to accelerate soft tissue maturation in esthetic periodontal surgery. Compend Contin Educ Dent 2001;22:729-32.

Dodson TT. Is there a role for reconstructive techniques to prevent periodontal defects after third molar surgery? Oral Maxillofac Surg Clin North Am 2007;19:99-104.

Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhgi J, et al. Platelet-rich fibrin (PRI): A second-generation platelet concentrate. Part I: Technological concepts and evolution. Oral Surg Oral Pathol Oral Radiol Endod 2006;101:37-44.

Ruga E, Gallesio C, Boffano P. Platelet-rich fibrin and piezoelectric surgery: A safe technique for the prevention of periodontal complications in third molar surgery. J Craniofac Surg 2011;22:1951-5.

Sammartino G, Tia M, Marenzi G, di Lauro AE, D’Agostino E, Claudio PP. Use of autologous platelet-rich plasma (PRP) in periodontal defect treatment after extraction of impacted mandibular third molars. J Oral Maxillofac Surg 2005;63:766-70.

Karapataki S, Hugoson A, Kugelberg CF. Healing following GTR treatment of bone defects distal to mandibular 2nd molars after surgical removal of impacted 3rd molars. J Clin Periodontol 2000;27:325-32.

Wiltfang J, Schlegel KA, Schultze-Mosgau S, Nkenke E, Zimmermann R, Kessler P. Sinus floor augmentation with beta-tricalciumphosphate (beta-TCP): Does platelet-rich plasma promote its osseous integration and degradation? Clin Oral Implants Res 2003;14:213-8.

Kassolis JD, Rosen PS, Reynolds MA. Alveolar ridge and sinus augmentation utilizing platelet-rich plasma in combination with freeze-dried bone allograft: Case series. J Periodontol 2000;71:1654-61.

Butterfield KJ, Bennett J, Gronowicz G, Adams D. Effect of platelet-rich plasma with autogenous bone graft for maxillary sinus augmentation in a rabbit model. J Oral Maxillofac Surg 2005;63:370-6.

Aghaloo TL, Moy PK, Freymiller EG. Evaluation of platelet-rich plasma in combination with anorganic bovine bone in the rabbit cranium: A pilot study. Int J Oral Maxillofac Implants 2004;19:59-65.

Zechner W, Tangl S, Tepper G, Fürst G, Bernhart T, Haas R, et al. Influence of platelet-rich plasma on osseous healing of dental implants: A histologic and histomorphometric study in minipigs. Int J Oral Maxillofac Implants 2003;18:15-22.

Choukroun J, Diss A, Simonpieri A, Girard MO, Schoeffler C, Dohan SL, et al. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part V: Histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:299-303.

Simonpieri A, Choukroun J, Girard MO, Ouaknine T, Dohan D. Immediate post-extraction implantation: Interest of the PRF. Implantodontie 2004;13:177-89.


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